Raises exception if not exactly one item:
Sequence unpacking:
singleitem, = mylist
# Identical in behavior (byte code produced is the same),
# but arguably more readable since a lone trailing comma could be missed:
[singleitem] = mylist
Rampant insanity, unpack the input to the identity lambda
function:
# The only even semi-reasonable way to retrieve a single item and raise an exception on
# failure for too many, not just too few, elements as an expression, rather than a
# statement, without resorting to defining/importing functions elsewhere to do the work
singleitem = (lambda x: x)(*mylist)
All others silently ignore spec violation, producing first or last item:
Explicit use of iterator protocol:
singleitem = next(iter(mylist))
Destructive pop:
singleitem = mylist.pop()
Negative index:
singleitem = mylist[-1]
Set via single iteration for
(because the loop variable remains available with its last value when a loop terminates):
for singleitem in mylist: break
There are many others (combining or varying bits of the above, or otherwise relying on implicit iteration), but you get the idea.