How do I create a list of random numbers without duplicates?

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This will return a list of 10 numbers selected from the range 0 to 99, without duplicates.

import random
random.sample(range(100), 10)

With reference to your specific code example, you probably want to read all the lines from the file once and then select random lines from the saved list in memory. For example:

all_lines = f1.readlines()
for i in range(50):
    lines = random.sample(all_lines, 40)

This way, you only need to actually read from the file once, before your loop. It’s much more efficient to do this than to seek back to the start of the file and call f1.readlines() again for each loop iteration.

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