How to iterate over the file in python

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The traceback indicates that probably you have an empty line at the end of the file.
You can fix it like this:

f = open('test.txt','r')
g = open('test1.txt','w') 
while True:
    x = f.readline()
    x = x.rstrip()
    if not x: break
    print >> g, int(x, 16)

On the other hand it would be better to use for x in f instead of readline. Do not forget to close your files or better to use with that close them for you:

with open('test.txt','r') as f:
    with open('test1.txt','w') as g: 
        for x in f:
            x = x.rstrip()
            if not x: continue
            print >> g, int(x, 16)

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