How to populate second dropdown based on selection of first dropdown using jQuery/AJAX and PHP/MySQL?

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First, your document-ready looks a bit off, it should either be $(document).ready(function(){}); or it could be just $(function(){});.

Second, you looping over the JSON result looks a bit odd as well. Try something like this instead:

$.each(data.subjects, function(i, val){    
   $('select#item_2').append('<option value="' + val.id + '">' + val.name + '</option>');
});

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