You can use column indices (letters) like this:
import pandas as pd
import numpy as np
file_loc = "path.xlsx"
df = pd.read_excel(file_loc, index_col=None, na_values=['NA'], usecols="A,C:AA")
print(df)
usecols : int, str, list-like, or callable default None
If None, then parse all columns.
If str, then indicates comma separated list of Excel column letters and column ranges (e.g. “A:E” or “A,C,E:F”). Ranges are inclusive of both sides.
If list of int, then indicates list of column numbers to be parsed.
If list of string, then indicates list of column names to be parsed.
New in version 0.24.0.
If callable, then evaluate each column name against it and parse the column if the callable returns True.
Returns a subset of the columns according to behavior above.
New in version 0.24.0.