Is it possible to make an ASP.NET MVC route based on a subdomain?

You can do it by creating a new route and adding it to the routes collection in RegisterRoutes in your global.asax. Below is a very simple example of a custom Route:

public class ExampleRoute : RouteBase
{

    public override RouteData GetRouteData(HttpContextBase httpContext)
    {
        var url = httpContext.Request.Headers["HOST"];
        var index = url.IndexOf(".");

        if (index < 0)
            return null;

        var subDomain = url.Substring(0, index);

        if (subDomain == "user1")
        {
            var routeData = new RouteData(this, new MvcRouteHandler());
            routeData.Values.Add("controller", "User1"); //Goes to the User1Controller class
            routeData.Values.Add("action", "Index"); //Goes to the Index action on the User1Controller

            return routeData;
        }

        if (subDomain == "user2")
        {
            var routeData = new RouteData(this, new MvcRouteHandler());
            routeData.Values.Add("controller", "User2"); //Goes to the User2Controller class
            routeData.Values.Add("action", "Index"); //Goes to the Index action on the User2Controller

            return routeData;
        }

        return null;
    }

    public override VirtualPathData GetVirtualPath(RequestContext requestContext, RouteValueDictionary values)
    {
        //Implement your formating Url formating here
        return null;
    }
}