Write OpenCV image in memory to BytesIO or Tempfile

cv2.imencode may help you:

import numpy as np
import cv2
import io

img = np.ones((100, 100), np.uint8)

# encode
is_success, buffer = cv2.imencode(".jpg", img)
io_buf = io.BytesIO(buffer)

# decode
decode_img = cv2.imdecode(np.frombuffer(io_buf.getbuffer(), np.uint8), -1)

print(np.allclose(img, decode_img))   # True

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