Can I have a route53 subdomain in a different Hosted Zone?

Create a hosted zone for test.example.com.

Note the 4 name servers that Route 53 assigns to it the new hosted zone.

Back in the master zone, create a new resource record, with hostname “test” using record type NS, and enter the 4 name servers that Route 53 assigned, in the box below.

You just delegated the “test.example.com” subdomain, and any hosts and subdomains in it, to a different hosted zone, which you can now break to your heart’s content, without impacting the production records.

The hosted zone for the subdomain can be in the same account or a different account.

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