Assuming your file is a WAV:
from tkinter import *
from winsound import *
root = Tk() # create tkinter window
play = lambda: PlaySound('Sound.wav', SND_FILENAME)
button = Button(root, text="Play", command = play)
button.pack()
root.mainloop()
Assuming your file is a MP3:
from Tkinter import *
import mp3play
root = Tk() # create tkinter window
f = mp3play.load('Sound.mp3'); play = lambda: f.play()
button = Button(root, text="Play", command = play)
button.pack()
root.mainloop()