You could use the ast lib:
from ast import literal_eval
df.Col3 = df.Col3.apply(literal_eval)
print(df.Col3[0][0])
Proj1
You can also do it when you create the dataframe from the csv, using converters
:
df = pd.read_csv("in.csv",converters={"Col3": literal_eval})
If you are sure the format is he same for all strings, stripping and splitting will be a lot faster:
df = pd.read_csv("in.csv",converters={"Col3": lambda x: x.strip("[]").split(", ")})
But you will end up with the strings wrapped in quotes