How to update matplotlib’s imshow() window interactively?

You don’t need to call imshow all the time. It is much faster to use the object’s set_data method:

myobj = imshow(first_image)
for pixel in pixels:
    addpixel(pixel)
    myobj.set_data(segmentedimg)
    draw()

The draw() should make sure that the backend updates the image.

UPDATE: your question was significantly modified. In such cases it is better to ask another question. Here is a way to deal with your second question:

Matplotlib’s animation only deals with one increasing dimension (time), so your double loop won’t do. You need to convert your indices to a single index. Here is an example:

import numpy as np
from matplotlib import pyplot as plt
from matplotlib import animation

nx = 150
ny = 50

fig = plt.figure()
data = np.zeros((nx, ny))
im = plt.imshow(data, cmap='gist_gray_r', vmin=0, vmax=1)

def init():
    im.set_data(np.zeros((nx, ny)))

def animate(i):
    xi = i // ny
    yi = i % ny
    data[xi, yi] = 1
    im.set_data(data)
    return im

anim = animation.FuncAnimation(fig, animate, init_func=init, frames=nx * ny,
                               interval=50)