Is it possible to run function in a subprocess without threading or writing a separate file/script.

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I think you’re looking for something more like the multiprocessing module:

http://docs.python.org/library/multiprocessing.html#the-process-class

The subprocess module is for spawning processes and doing things with their input/output – not for running functions.

Here is a multiprocessing version of your code:

from multiprocessing import Process, Queue

# must be a global function    
def my_function(q, x):
    q.put(x + 100)

if __name__ == '__main__':
    queue = Queue()
    p = Process(target=my_function, args=(queue, 1))
    p.start()
    p.join() # this blocks until the process terminates
    result = queue.get()
    print result

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