It is not really well formated question … You should be more precise about what you want, what you are using, etc.
Assuming you are using Rails 3, ERB for HTML generation:
# in your controller:
file_as_array = IO.readlines('filename.txt')
random_line = rand(file_as_array.size)
@my_random_string = file_as_array[random_line]
# in your view:
<%= @my_random_string %>