Tuples are immutable, you may not change their contents.
With a list
>>> x = [1,2,3]
>>> x[0] = 5
>>> x
[5, 2, 3]
With a tuple
>>> y = tuple([1,2,3])
>>> y
(1, 2, 3)
>>> y[0] = 5 # Not allowed!
Traceback (most recent call last):
File "<pyshell#20>", line 1, in <module>
y[0] = 5
TypeError: 'tuple' object does not support item assignment
But if I understand your question, say you have
>>> a = [1,2,3]
>>> b = [4,5,6]
>>> t = (a,b)
>>> t
([1, 2, 3], [4, 5, 6])
You are allowed to modify the internal lists as
>>> t[0][0] = 5
>>> t
([5, 2, 3], [4, 5, 6])