When you are using the unsorted list all tuples are accessed in memory-order. They have been allocated consecutively in RAM. CPUs love accessing memory sequentially because they can speculatively request the next cache line so it will always be present when needed.
When you are sorting the list you put it into random order because your sort keys are randomly generated. This means that the memory accesses to tuple members are unpredictable. The CPU cannot prefetch memory and almost every access to a tuple is a cache miss.
This is a nice example for a specific advantage of GC memory management: data structures which have been allocated together and are used together perform very nicely. They have great locality of reference.
The penalty from cache misses outweighs the saved branch prediction penalty in this case.
Try switching to a struct-tuple. This will restore performance because no pointer-dereference needs to occur at runtime to access tuple members.
Chris Sinclair notes in the comments that “for TotalCount around 10,000 or less, the sorted version does perform faster“. This is because a small list fits entirely into the CPU cache. The memory accesses might be unpredictable but the target is always in cache. I believe there is still a small penalty because even a load from cache takes some cycles. But that seems not to be a problem because the CPU can juggle multiple outstanding loads, thereby increasing throughput. Whenever the CPU hits a wait for memory it will still speed ahead in the instruction stream to queue as many memory operations as it can. This technique is used to hide latency.
This kind of behavior shows how hard it is to predict performance on modern CPUs. The fact that we are only 2x slower when going from sequential to random memory access tell me how much is going on under the covers to hide memory latency. A memory access can stall the CPU for 50-200 cycles. Given that number one could expect the program to become >10x slower when introducing random memory accesses.