Splitting a pandas dataframe column by delimiter

Use vectoried str.split with expand=True:

In [42]:
df[['V','allele']] = df['V'].str.split('-',expand=True)
df

Out[42]:
      ID    Prob      V allele
0   3009  1.0000  IGHV7   B*01
1    129  1.0000  IGHV7   B*01
2    119  0.8000  IGHV6   A*01
3    120  0.8056   GHV6   A*01
4    121  0.9000  IGHV6   A*01
5    122  0.8050  IGHV6   A*01
6    130  1.0000  IGHV4   L*03
7   3014  1.0000  IGHV4   L*03
8    266  0.9970  IGHV5   A*01
9    849  0.4010  IGHV5   A*04
10   174  1.0000  IGHV6   A*02
11   844  1.0000  IGHV6   A*02